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FASTQ Analysis Results

Excellent Quality

What This Means

This sample data demonstrates excellent quality sequencing results!

5
Total Sequences ?
35
Average Length (bp) ?
Q38
Average Quality Score ?
45.7%
GC Content ?
Understanding DNA Sequencing Quality

Quality Scores (Q-scores): Measure the accuracy of each DNA letter (base) in your sequences.

Quality Distribution
Excellent (Q30+)
5
100.0%
Good (Q20-29)
0
0.0%
Fair (Q10-19)
0
0.0%
Poor (Q0-9)
0
0.0%
Length Distribution
0-50bp
5
100.0%
51-100bp
0
0.0%
101-150bp
0
0.0%
151-200bp
0
0.0%
200+bp
0
0.0%
Base Composition
A
37
25.5%
T
38
26.2%
G
35
24.1%
C
35
24.1%

Quality Control Visualizations

Per Base Sequence Quality

This plot shows the distribution of quality scores at each position in the read. Box plots represent quartiles, with whiskers extending to the 10th and 90th percentiles.

Per Sequence Quality Scores

Distribution of mean quality scores across all sequences.

Per Base Sequence Content

The proportion of each base (A, T, G, C) at each position across all sequences.

A T G C

Sample Sequences (Showing 5 of 5)

SEQ_ID_001
35 bp ✓ Q40
GATTACAGATTACAGATTACAGATTACAGATTACA
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
Average quality: Excellent (Q40)
SEQ_ID_002
35 bp ✓ Q39
CGATCGATCGATCGATCGATCGATCGATCGATCGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
Average quality: Excellent (Q39)
SEQ_ID_003
35 bp ✓ Q38
ATCGATCGATCGATCGATCGATCGATCGATCGATC
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
Average quality: Excellent (Q38)
SEQ_ID_004
34 bp ✓ Q37
TACGTACGTACGTACGTACGTACGTACGTACGTA
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFH
Average quality: Excellent (Q37)
SEQ_ID_005
34 bp ✓ Q36
GCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGC
EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
Average quality: Excellent (Q36)

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